bioinformatics BLAST Create a DNA blastdb
Example
In order to compare query sequences against reference sequences, you must create a blastdb of your reference(s). This is done using makeblastdb which is included when you install blast.
makeblastdb -in <input fasta> -dbtype nucl -out <label for database>
So if you had a file reference.fasta containing the following records:
>reference_1
ATCGATAAA
>reference_2
ATCGATCCC
You would run the following:
makeblastdb -in reference.fasta -dbtype nucl -out my_database
This would create the following files:
my_database.nhrmy_database.ninmy_database.nsq
Note, the database files are labelled with the -out argument.
